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Genomics Core is equipped for high-throughput DNA/RNA sequencing experiments including a NextSeq 550, 10X Genomics Chromium System, 4200 TapeStation, BioRad qPCR machine, digital PCR system.
Taegun Kwon, PhD | Manager | 817-735-0614 | Genomics.Core@unthsc.edu
Location and hours of operation
| Hours | Location |
|
Monday - Friday 8AM - 5 PM |
3500 Camp Bowie Blvd, Fort Worth. TX 76107 Lab & Office: RES-126 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Taegun Kwon, PhD |
Core Manager
|
817-735-0614
|
Taegun.Kwon@unthsc.edu
|
RES-126
|
| Sharad Shrestha, PhD |
Research Core Labs Director
|
817-735-0117
|
Sharad.Shrestha@unthsc.edu
|
| Service list |
| ► RNA Sequencing Library Prep (1) | |||
| Name | Description | Price | |
|---|---|---|---|
| RNA sequencing library prep |
The common RNA sequencing library prep methods include: Stranded mRNA sequencing library prep Stranded total RNA sequencing library prep Small RNA sequencing library prep RNA sequencing has more than 30 variations in protocol. Please, log in and inquire for more information. |
Inquire | |
| ► 10x Chromium System (2) | |||
| Name | Description | Price | |
| 10x Sample preparation - Dead cell removal |
Enrichment of live cells from single cell suspension using the Miltenyi Dead Cell Removal Kit |
Inquire | |
| Single Cell Immune Profiling |
Chromium Single Cell Immune Profiling provides a multiomic solution to your immunology questions. Analyze full length paired B-cell or T-cell receptors, surface protein expression, antigen specificity, and gene expression, all from a single cell.
Note that the cost for single cell sequencing library prep varies by configuration. Please, request for a quote for accurate pricing. |
Inquire | |
| ► Epigenetic services (5) | |||
| Name | Description | Price | |
| Antibody for ChIP: Androgen Receptor pAb |
Cost per reaction. Rabbit derived. |
Inquire | |
| Antibody for ChIP: Histone H3K27ac (rAb) |
Cost per reaction. Mouse derived. |
Inquire | |
| Antibody for ChIP: Histone H3K27me3 pAb |
Cost per reaction. Rabbit derived. |
Inquire | |
| ATAC-seq |
ATAC-Seq is a rapid assay of the epigenetic state by enabling identification of open chromatin regions. In the assay, intact nuclei are treated with a hyperactive Tn5 transposase mutant which is able to simultaneously tag the target DNA with sequencing adapters and frag- ment the DNA in a process termed “tagmentation”2. Because of the assay’s speed, simplicity, sensitivity, and applicability to a wide range of sample types, ATAC-Seq has become a commonly used epigentic assay, and can serve as a gateway to further, more detailed, epigenetic analyses. The ATAC-Seq Kit provides the reagents necessary to produce 16 unique sequencing-ready Illumina®-compatible ATAC-Seq libraries from 20 - 30 mg tissue or 50,000 - 100,000 cells per reaction. |
Inquire | |
| ChIP sample prep |
Designed to allow high quality DNA enrichment from as little as 1,000 cells per ChIP reaction for abundant targets and as little as 50,000 cells for low abundance transcription factors. The kit is also optimized to give superior results when performing chromatin immunoprecipitation using antibodies against low abundance proteins, such as transcription factors, or antibodies with sub-optimal binding affinities.
Does not include antibody. Please see list of antibodies available in epigenetic services. |
Inquire | |
| ► MiSeq Sequencing (8) | |||
| Name | Description | Price | |
| MiSeq 15M reads - 300 cycles | Inquire | ||
| MiSeq 15M reads - 50 cycles | Inquire | ||
| MiSeq 15M reads - 500 cycle | Inquire | ||
| MiSeq 1M reads - 300 cycle | Inquire | ||
| MiSeq 1M reads - 500 cycles | Inquire | ||
| MiSeq 25M reads - 150 cycles | Inquire | ||
| MiSeq 25M reads - 600 cycles | Inquire | ||
| MiSeq 4M reads - 300 cycle | Inquire | ||
| ► NextSeq Sequencing (5) | |||
| Name | Description | Price | |
| NextSeq 550 High Output kit (150 cycles) |
Output is 400M read pairs; Option for 75bp PE reads or 150bp SE reads available. Recommended for: whole transcriptome, mRNA-seq, and exome sequencing.
|
Inquire | |
| NextSeq 550 High Output kit (300 cycles) |
Maximum output: 120Gb. Maximum reads per run: 400 million clusters. Option for 150x150bp PE reads or 300bp SE reads available. Recommended for whole genome, de novo, and exome sequencing.
|
Inquire | |
| NextSeq 550 High Output kit (75 cycles) |
Maximum output: 60Gb Maximum reads per run: 400 million clusters Recommended for: gene expression profiling and NIPT.
|
Inquire | |
| NextSeq 550 Mid Output kit (150 cycles) |
Maximum output: 60Gb Maximum reads per run: 130 million clusters Options for 75x75bp PE reads or 150bp SE reads available. Recommended for: Whole transcriptome, mRNA-seq, and exome sequencing.
|
Inquire | |
| NextSeq 550 Mid Output kit (300 cycles) |
Maximum output: 60Gb Maximum reads per run: 130 million clusters Options for 150x150bp PE reads or 300bp SE reads available. Recommended for: targeted panels and exome sequencing.
|
Inquire | |
| ► NovaSeq Sequencing w/ NTGC (4) | |||
| Name | Description | Price | |
| Per Flow Cell - SP 1x100 |
800M reads |
Inquire | |
| Per Flow Cell - SP 2x100 |
800M reads |
Inquire | |
| S4 2x150 (whole lane) | Inquire | ||
| SP Flow Cell 300 cycle | Inquire | ||
| ► TapeStation 4200 (1) | |||
| Name | Description | Price | |
| Automated electrophoresis system |
The 4200 TapeStation system is a high-throughput automated electrophoresis platform for nucleic acid sample quality control.
The available analyses include: RNA ScreenTape Assay D1000 ScreenTape Assay D5000 ScreenTape Assay Genomic DNA ScreenTape Assay Cell-free DNA ScreenTape Assay
Please, log in to view more information. |
Inquire | |
| ► Biological Material Testing (8) | |||
| Name | Description | Price | |
| CellCheck 16 - Human |
This is through our collaboration with IDEXX Includes price of shipping -- please include styrofoam box within a cardboard box for shipping if you already have on on hand, otherwise we can provide one for you.
|
Inquire | |
| CellCheck 16 Plus - Human |
This is through our collaboration with IDEXX Includes price of shipping -- please include styrofoam box within a cardboard box for shipping if you already have on on hand, otherwise we can provide one for you. Includes everything in the CellCheck 16-Human service and Mycoplasma spp Testing |
Inquire | |
| CellCheck 9 - Human |
This is through our collaboration with IDEXX Includes price of shipping -- please include styrofoam box within a cardboard box for shipping if you already have on on hand, otherwise we can provide one for you.
|
Inquire | |
| CellCheck 9 Plus - Human |
This is through our collaboration with IDEXX Includes price of shipping -- please include styrofoam box within a cardboard box for shipping if you already have on on hand, otherwise we can provide one for you. Includes everything in the CellCheck 9-Human service and Mycoplasma spp Testing |
Inquire | |
| CellCheck Mouse 19 |
This is through our collaboration with IDEXX Includes price of shipping -- please include styrofoam box within a cardboard box for shipping if you already have on on hand, otherwise we can provide one for you.
|
Inquire | |
| CellCheck Mouse 19 Plus |
This is through our collaboration with IDEXX Includes price of shipping -- please include styrofoam box within a cardboard box for shipping if you already have on on hand, otherwise we can provide one for you. Includes everything in the CellCheck Mouse 19 service and Mycoplasma spp Testing |
Inquire | |
| IMPACT II and C. Boris Bundle |
This is through our collaboration with IDEXX Includes price of shipping -- please include styrofoam box within a cardboard box for shipping if you already have on on hand, otherwise we can provide one for you. Includes: Mycoplasma spp, M. pulmonis, MHV, MVM, MPV 1-5, TMEV, Sendai, PVM, MNV, |
Inquire | |
| STAT-Mycoplasma spp. PCR Testing |
This is through our collaboration with IDEXX Includes price of shipping -- please include styrofoam box within a cardboard box for shipping if you already have on on hand, otherwise we can provide one for you. |
Inquire | |
| ► Extractions (2) | |||
| Name | Description | Price | |
| DNA extraction |
Extraction of DNA using a standard PCI protocol. FFPE/embalmed samples accepted but quality not guaranteed. |
Inquire | |
| RNA Extractions |
Per sample |
Inquire | |
| ► PCR-Based Assay: Digital PCR / Quantitative PCR / Realtime PCR (3) | |||
| Name | Description | Price | |
| Digital PCR Assay |
Use the [Schedule Equipment] tab if you are trained to operate the instrument by yourself. Pick up reagents and consumables at the Genomics core and take advantages of bulk pricing.
Fill out this form only to request a digital PCR assay performed by a core staff. Note 1. We perform digital PCR assay in the set of 4 reactions; hence, it will be billed by the unit of 4 reactions. Note 2. Each instrument run may hold up to 16 reactions. The instrument run charge is added to the bill every 16 reactions. |
Inquire | |
| Quantitative real-time PCR (qPCR) |
Universal SYBR® qPCR (price per reaction) |
Inquire | |
| cDNA synthesis for quantitative PCR | Inquire | ||
